Yeast is the essential raw material for brewing beer, and it has a significant impact on the flavor of beer. There are two methods of adding yeast in the beer brewing process: direct addition and activation through a yeast propagator. Now I will introduce to you the commonly used yeast activation method in Medell’s small-scale beer equipment.
1. Before starting the operation, place the strain in a cryovial at room temperature. Once the internal medium reaches room temperature, shake it evenly. For aseptic operation, transfer all the culture solution from the cryovial into a test tube containing 5 mL of culture medium. Incubate it at 25±1°C for 72±2 hours.
2. Gently shake the culture medium in the test tube to distribute it evenly. Using an inoculating loop, pick up three rings of yeast and add them to another test tube containing 5 mL of medium. Incubate it at 25±1°C for 24-36 hours.
3. After completing the culture, gently shake the culture medium in the test tube. Transfer 0.1 mL of the test tube to 10 mL of medium using a sterile pipette. Shake it evenly and incubate at 25±1°C for 24-36 hours.
That’s the yeast activation method. Once the yeast is activated, it can be added to the beer fermentation tank.
I hope this information is helpful to you. If you have any questions or suggestions regarding the selection of beer equipment or the beer brewing process, feel free to leave a message to ACE beer equipment.